A full thickness wound 1.5cm x 1.5cm is incised and excised on both sides of the spine. Following excisions, doxorubicin is injected to the peripheral edges of the wounds. After few days, doxorubicin is reinjected to the peripheral edges of the wounds. From day 8 forwarded the wounds are checked daily for evaluation of the eschar formation on the wound. Treatment begin after all wounds developed eschar.
Pig skin has been shown to be the most similar to human skin structurally and biochemically.
Pigs are especially useful in wound healing and burn lesions studies due to the re-revitalization healing (as human) rather than by contraction (as rodent). Another advantage is the ability to use multiple wounds on the same animal which provides the ability to have the same animal serve as its own control and also to have different treatment on the same animal.
This model was used by companies to estimate the dose and treatment duration for maximal efficacy of eschar removal in chronic wounds.